Peptide Database

Peptide Namevpr signal peptide

Function Ontology

Annotation / Sequence feature ▸ Signal peptide

Biotechnology / Production and expression ▸ Secretory enzyme production ▸ Alkaline protease secretion

Biotechnology / Production and expression ▸ Secretory expression optimization

Standard StatusStandard

Sequence Length29 amino acids

Chemical FormulaC150H243N35O35S

DescriptionStandard

        MKKGIIRFLLVSFVLFFALSTGITGVQAA
    

Molecular Weight3128.81 Da

Isoelectric Point (pI)11.17

Net Charge (pH 7.0)2.50

Net Charge (pH 7.4) 2.28

Charge Density (pH 7.0) 0.0861

GRAVY Index1.37

Hydrophobic Moment 0.48

Boman Index0.46

Instability Index-4.98

Aliphatic Index 134.48

Aromaticity 0.1379

Extinction Coeff. Reduced 0.00

Extinction Coeff. Oxidized 0.00

The radar chart summarizes major physicochemical dimensions for quick comparison, while exact numerical values remain listed on the left.

Amino Acid Composition

TPSA 1116.07

LogP -5.53

H-bond Donors 40.0

H-bond Acceptors 39.0

Rotatable Bonds 104.0

Heavy Atoms 221.0

Ring Count 4.0

Amide Bonds 29.0

3D PDB MODEL

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2D CHEMICAL STRUCTURE

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RECORD: Rec_0354960 VERIFIED: YES

Provenance & Taxonomy

OrganismBacillus subtilis subsp. subtilis str. 168 [Bacterium]

FamilySec signal peptide

Tax ID224308

Structure & Mods

Configlinear signal peptide [Linear]

Chiralitycanonical L-amino-acid peptide [L]

Bio-Activity Profile

Function

Protein secretion signal peptide Secretory expression optimization Alkalineprotease (alk) secretion

TargetBacillus subtilis WB600

Activity2369.89 U/mL

MethodProtease activity assay

PaperLiu Y, Shi C, Li D, et al. Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide[J]. International journal of biological macromolecules, 2019, 138: 903-911. PMID31356949 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31356949/