Peptide Database

Peptide NameynfF signal peptide

Function Ontology

Annotation / Sequence feature ▸ Signal peptide

Biotechnology / Production and expression ▸ Secretory enzyme production ▸ Alkaline protease secretion

Biotechnology / Production and expression ▸ Secretory expression optimization

Standard StatusStandard

Sequence Length46 amino acids

Chemical FormulaC216H367N61O63S4

DescriptionStandard

        MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVRAA
    

Molecular Weight4954.86 Da

Isoelectric Point (pI)11.00

Net Charge (pH 7.0)3.59

Net Charge (pH 7.4) 3.32

Charge Density (pH 7.0) 0.0780

GRAVY Index0.31

Hydrophobic Moment 0.48

Boman Index0.10

Instability Index60.29

Aliphatic Index 91.52

Aromaticity 0.0435

Extinction Coeff. Reduced 0.00

Extinction Coeff. Oxidized 0.00

The radar chart summarizes major physicochemical dimensions for quick comparison, while exact numerical values remain listed on the left.

Amino Acid Composition

TPSA 1996.69

LogP -21.36

H-bond Donors 73.0

H-bond Acceptors 72.0

Rotatable Bonds 169.0

Heavy Atoms 344.0

Ring Count 4.0

Amide Bonds 46.0

3D PDB MODEL

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2D CHEMICAL STRUCTURE

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RECORD: Rec_0244850 VERIFIED: YES

Provenance & Taxonomy

OrganismEscherichia coli K-12 [Bacterium]

FamilySec signal peptide

Tax ID83333

Structure & Mods

Configlinear signal peptide [Linear]

Chiralitycanonical L-amino-acid peptide [L]

Bio-Activity Profile

Function

Protein secretion signal peptide Secretory expression optimization Alkalineprotease (alk) secretion

TargetBacillus subtilis WB600

Activity1815.64 U/mL

MethodProtease activity assay

PaperLiu Y, Shi C, Li D, et al. Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide[J]. International journal of biological macromolecules, 2019, 138: 903-911. PMID31356949 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31356949/