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MYP_158906
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Basic Information
vpr-2 signal peptide
Annotation / Sequence feature ▸ Signal peptide
Biotechnology / Production and expression ▸ Secretory expression optimization
Enzyme / Catalytic activity ▸ Hydrolase ▸ Protease/Peptidase
Enzyme / Catalytic activity ▸ Lyase
Standard
28 amino acids
C147H238N34O34S
Standard
Sequence
MKKGIIRFLLVSFVLFFALSTGITGVQA
Physicochemical Analysis
3057.73 Da
11.17
2.50
2.28
0.0892
1.36
0.48
0.46
-5.52
135.71
0.1429
0.00
0.00
The radar chart summarizes major physicochemical dimensions for quick comparison, while exact numerical values remain listed on the left.
Residue Composition
Amino Acid Composition
Chemical Descriptors
1086.97
-5.03
39.0
38.0
102.0
216.0
4.0
28.0
Structural Visualization
3D PDB MODEL
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2D CHEMICAL STRUCTURE
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Experimental Records
RECORD: Rec_0043686 VERIFIED: YES
Provenance & Taxonomy
Bacillus subtilis [Bacterium]
Sec signal peptide
1423
Structure & Mods
linear signal peptide [Linear]
canonical L-amino-acid peptide [L]
Bio-Activity Profile
Protein secretion signal peptide Secretory expression optimization L-Asparaginase secretion
Bacillus subtilis WB600
0.0 U/mL
Asparaginase activity assay
PaperXuan Chen.Study on the expression and fermentation optimization of L-asparaginase in Bacillus subtilis WB600[D]. Jiangnan University, 2015. (in Chinese)
RECORD: Rec_0281025 VERIFIED: YES
Provenance & Taxonomy
Bacillus subtilis [Bacterium]
Sec signal peptide
1423
Structure & Mods
linear signal peptide [Linear]
canonical L-amino-acid peptide [L]
Bio-Activity Profile
Protein secretion signal peptide Secretory expression optimization Aminopeptidase (AP) secretion
Bacillus subtilis WB600
4.45 U/mL
Aminopeptidase activity assay
PaperGuan C, Cui W, Cheng J, et al. Construction of a highly active secretory expression system via an engineered dual promoter and a highly efficient signal peptide in Bacillus subtilis[J]. New Biotechnology, 2016, 33(3): 372-379. PMID26820123 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/26820123/
RECORD: Rec_0304304 VERIFIED: YES
Provenance & Taxonomy
Bacillus subtilis [Bacterium]
Sec signal peptide
1423
Structure & Mods
linear signal peptide [Linear]
canonical L-amino-acid peptide [L]
Bio-Activity Profile
Protein secretion signal peptide Secretory expression optimization Alkaline polygalacturonate lyase (PGL) secretion
Bacillus subtilis WB600
16.8 U/mL
Polygalacturonate lyase activity assay
PaperZhang J J, Kang Z, Ling Z M, et al. High-level extracellular production of alkaline polygalacturonate lyase in Bacillus subtilis with optimized regulatory elements[J]. Bioresource Technology, 2013, 146: 543-548 PMID23973973 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/23973973/