Peptide Database

Peptide NamePleu(i+4)1,15(A9K)

Function Ontology

Antimicrobial

Antimicrobial ▸ Antibacterial

Toxicity / Safety ▸ Blood cell toxicity ▸ Hemolysis ▸ Hemolytic

Standard StatusStandard

Sequence Length25 amino acids

Chemical FormulaC132H199N37O29

DescriptionStandard

        GWGSFFKKKAHVGKHVGKAALTHYL
    

Molecular Weight2768.22 Da

Isoelectric Point (pI)10.30

Net Charge (pH 7.0)5.02

Net Charge (pH 7.4) 4.65

Charge Density (pH 7.0) 0.2006

GRAVY Index-0.30

Hydrophobic Moment 0.53

Boman Index0.10

Instability Index4.97

Aliphatic Index 66.40

Aromaticity 0.1600

Extinction Coeff. Reduced 6990.00

Extinction Coeff. Oxidized 6990.00

The radar chart summarizes major physicochemical dimensions for quick comparison, while exact numerical values remain listed on the left.

Amino Acid Composition

TPSA 1054.34

LogP -6.90

H-bond Donors 38.0

H-bond Acceptors 37.0

Rotatable Bonds 91.0

Heavy Atoms 198.0

Ring Count 8.0

Amide Bonds 24.0

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber in the current view mode. Use the buttons to zoom.

2D CHEMICAL STRUCTURE

Drag to move

Drag to move. Use the buttons to zoom.

RECORD: Rec_0040274 VERIFIED: YES

Provenance & Taxonomy

Organismsynthetic construct [Synthetic]

Tax ID32630

Structure & Mods

Confighydrocarbon-stapled side-chain cyclic peptide [Cyclic]

Chiralitycanonical L-amino-acid peptide [L]

N-TermNone

C-TermAmidation

Other ModsThe Ⓧ (position: 2, 6, 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. Ⓧ (2) and Ⓧ (6), Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling respectively

Bio-Activity Profile

Function

Antimicrobial Antibacterial

TargetEscherichia coli ATCC 25922

Activity1.56 μg/mL

MethodMIC

PaperRida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky. Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice. Nat Biotechnol. 2019 Oct;37(10):1186-1197. Epub 2019 Aug 19. PMID31427820 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31427820/

RECORD: Rec_0163882 VERIFIED: YES

Provenance & Taxonomy

Organismsynthetic construct [Synthetic]

Tax ID32630

Structure & Mods

Confighydrocarbon-stapled side-chain cyclic peptide [Cyclic]

Chiralitycanonical L-amino-acid peptide [L]

N-TermNone

C-TermAmidation

Other ModsThe Ⓧ (position: 2, 6, 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. Ⓧ (2) and Ⓧ (6), Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling respectively

Bio-Activity Profile

Function

Antimicrobial Antibacterial

TargetStaphylococcus aureus ATCC 25923

Activity3.13 μg/mL

MethodMIC

PaperRida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky. Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice. Nat Biotechnol. 2019 Oct;37(10):1186-1197. Epub 2019 Aug 19. PMID31427820 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31427820/

RECORD: Rec_0209637 VERIFIED: YES

Provenance & Taxonomy

Organismsynthetic construct [Synthetic]

Tax ID32630

Structure & Mods

Confighydrocarbon-stapled side-chain cyclic peptide [Cyclic]

Chiralitycanonical L-amino-acid peptide [L]

N-TermNone

C-TermAmidation

Other ModsThe Ⓧ (position: 2, 6, 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. Ⓧ (2) and Ⓧ (6), Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling respectively

Bio-Activity Profile

Function

Antimicrobial Antibacterial

TargetPseudomonas aeruginosa ATCC 27853

Activity1.56 μg/mL

MethodMIC

PaperRida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky. Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice. Nat Biotechnol. 2019 Oct;37(10):1186-1197. Epub 2019 Aug 19. PMID31427820 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31427820/

RECORD: Rec_0319415 VERIFIED: YES

Provenance & Taxonomy

Organismsynthetic construct [Synthetic]

Tax ID32630

Structure & Mods

Confighydrocarbon-stapled side-chain cyclic peptide [Cyclic]

Chiralitycanonical L-amino-acid peptide [L]

N-TermNone

C-TermAmidation

Other ModsThe Ⓧ (position: 2, 6, 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. Ⓧ (2) and Ⓧ (6), Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling respectively

Bio-Activity Profile

Function

Antimicrobial Antibacterial

TargetBacillus cereus ATCC 14579

Activity3.13 μg/mL

MethodMIC

PaperRida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky. Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice. Nat Biotechnol. 2019 Oct;37(10):1186-1197. Epub 2019 Aug 19. PMID31427820 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31427820/

RECORD: Rec_0319779 VERIFIED: YES

Provenance & Taxonomy

Organismsynthetic construct [Synthetic]

Tax ID32630

Structure & Mods

Confighydrocarbon-stapled side-chain cyclic peptide [Cyclic]

Chiralitycanonical L-amino-acid peptide [L]

N-TermNone

C-TermAmidation

Other ModsThe Ⓧ (position: 2, 6, 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. Ⓧ (2) and Ⓧ (6), Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling respectively

Bio-Activity Profile

Function

Hemolytic

Targethuman red blood cells

Activity25 μg/mL

MethodHemolysis

PaperRida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky. Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice. Nat Biotechnol. 2019 Oct;37(10):1186-1197. Epub 2019 Aug 19. PMID31427820 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31427820/