Peptide Database

Peptide NamebslA signal peptide

Function Ontology

Annotation / Sequence feature ▸ Signal peptide

Biotechnology / Production and expression ▸ Secretory enzyme production ▸ Cutinase secretion

Biotechnology / Production and expression ▸ Secretory expression optimization

Enzyme modulation ▸ Enzyme inhibition ▸ Amylase inhibition ▸ Alpha-amylase inhibitory

Standard StatusStandard

Sequence Length30 amino acids

Chemical FormulaC137H237N35O40S

DescriptionStandard

        MKRKLLSSLAISALSLGLLVSAPTASFAAE
    

Molecular Weight3046.62 Da

Isoelectric Point (pI)9.99

Net Charge (pH 7.0)1.51

Net Charge (pH 7.4) 1.28

Charge Density (pH 7.0) 0.0502

GRAVY Index0.92

Hydrophobic Moment 0.36

Boman Index0.29

Instability Index30.54

Aliphatic Index 133.67

Aromaticity 0.0333

Extinction Coeff. Reduced 0.00

Extinction Coeff. Oxidized 0.00

The radar chart summarizes major physicochemical dimensions for quick comparison, while exact numerical values remain listed on the left.

Amino Acid Composition

TPSA 1191.28

LogP -11.54

H-bond Donors 43.0

H-bond Acceptors 43.0

Rotatable Bonds 102.0

Heavy Atoms 213.0

Ring Count 2.0

Amide Bonds 29.0

3D PDB MODEL

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2D CHEMICAL STRUCTURE

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RECORD: Rec_0058471 VERIFIED: YES

Provenance & Taxonomy

OrganismBacillus subtilis [Bacterium]

FamilySec signal peptide

Tax ID1423

Structure & Mods

Configlinear signal peptide [Linear]

Chiralitycanonical L-amino-acid peptide [L]

Bio-Activity Profile

Function

Protein secretion signal peptide Secretory expression optimization alpha-amylase (AmyS) secretion

TargetBacillus subtilis 1A751p

Activity1153.4 U/mL

MethodAmylase activity assay

PaperFu G, Liu J L, Li J S, et al. Systematic Screening of Optimal Signal Peptides for Secretory Production of Heterologous Proteins in Bacillus subtilis[J]. Journal of Agricultural and Food Chemistry, 2018, 66(50): 13141-13151. PMID30463403 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/30463403/

RECORD: Rec_0068099 VERIFIED: YES

Provenance & Taxonomy

OrganismBacillus subtilis [Bacterium]

FamilySec signal peptide

Tax ID1423

Structure & Mods

Configlinear signal peptide [Linear]

Chiralitycanonical L-amino-acid peptide [L]

Bio-Activity Profile

Function

Protein secretion signal peptide Secretory expression optimization Cutinase secretion

TargetBacillus subtilis TEB1030

Activity0.0 U/mL

MethodCutinase activity assay

PaperBrockmeier U, Caspers M, Freudl R, et al. Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria[J]. Journal of Molecular Biology, 2006, 362(3): 393-402. PMID16930615 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/16930615/