Peptide Database

Peptide NamephoD signal peptide

Function Ontology

Annotation / Sequence feature ▸ Signal peptide

Biotechnology / Production and expression ▸ Secretory enzyme production ▸ Alkaline protease secretion

Biotechnology / Production and expression ▸ Secretory expression optimization

Standard StatusStandard

Sequence Length57 amino acids

Chemical FormulaC284H440N78O86S

DescriptionStandard

        MAYDSRFDEWVQKLKEESFQNNTFDRRKFIQGAGKIAGLSLGLTIAQSVGAFEVNAA
    

Molecular Weight6355.07 Da

Isoelectric Point (pI)6.11

Net Charge (pH 7.0)-0.49

Net Charge (pH 7.4) -0.72

Charge Density (pH 7.0) -0.0086

GRAVY Index-0.30

Hydrophobic Moment 0.72

Boman Index0.00

Instability Index38.89

Aliphatic Index 75.44

Aromaticity 0.1228

Extinction Coeff. Reduced 6990.00

Extinction Coeff. Oxidized 6990.00

The radar chart summarizes major physicochemical dimensions for quick comparison, while exact numerical values remain listed on the left.

Amino Acid Composition

TPSA 2702.83

LogP -27.62

H-bond Donors 93.0

H-bond Acceptors 87.0

Rotatable Bonds 217.0

Heavy Atoms 449.0

Ring Count 8.0

Amide Bonds 63.0

3D PDB MODEL

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2D CHEMICAL STRUCTURE

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RECORD: Rec_0058430 VERIFIED: YES

Provenance & Taxonomy

OrganismBacillus subtilis subsp. subtilis str. 168 [Bacterium]

FamilyTat signal peptide

Tax ID224308

Structure & Mods

Configlinear signal peptide [Linear]

Chiralitycanonical L-amino-acid peptide [L]

Bio-Activity Profile

Function

Protein secretion signal peptide Secretory expression optimization Alkalineprotease (alk) secretion

TargetBacillus amyloliquefaciens 111018

Activity10963.9 U/mL

MethodProtease activity assay

PaperLiu Y, Shi C, Li D, et al. Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide[J]. International journal of biological macromolecules, 2019, 138: 903-911. PMID31356949 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31356949/