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MYP_135553
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Basic Information
estA signal peptide
Annotation / Sequence feature ▸ Signal peptide
Biotechnology / Production and expression ▸ Secretory expression optimization
Enzyme / Catalytic activity ▸ Hydrolase ▸ Lipase
Enzyme modulation ▸ Enzyme inhibition ▸ Amylase inhibition ▸ Alpha-amylase inhibitory
Standard
32 amino acids
C163H282N42O39S2
Standard
Sequence
MKFVKRRIIALVTILMLSVTSLFALQPSAKAA
Physicochemical Analysis
3518.37 Da
12.00
4.50
4.28
0.1405
1.06
0.49
0.27
59.81
140.31
0.0625
0.00
0.00
The radar chart summarizes major physicochemical dimensions for quick comparison, while exact numerical values remain listed on the left.
Residue Composition
Amino Acid Composition
Chemical Descriptors
1302.73
-7.72
47.0
46.0
119.0
246.0
3.0
32.0
Structural Visualization
3D PDB MODEL
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2D CHEMICAL STRUCTURE
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Experimental Records
RECORD: Rec_0091139 VERIFIED: YES
Provenance & Taxonomy
Bacillus subtilis [Bacterium]
Sec signal peptide
1423
Structure & Mods
linear signal peptide [Linear]
canonical L-amino-acid peptide [L]
Bio-Activity Profile
Protein secretion signal peptide Secretory expression optimization Natto phytase secretion
Bacillus subtilis RIK1285
2.466 U/mL
Phytase activity assay
PaperTsuji S, Tanaka K, Takenaka S, et al. Enhanced secretion of natto phytase by Bacillus subtilis[J]. Bioscience Biotechnology and Biochemistry, 2015, 79(11): 1906-1914. PMID26023739 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/26023739/
RECORD: Rec_0328586 VERIFIED: YES
Provenance & Taxonomy
Bacillus subtilis [Bacterium]
Sec signal peptide
1423
Structure & Mods
linear signal peptide [Linear]
canonical L-amino-acid peptide [L]
Bio-Activity Profile
Protein secretion signal peptide Secretory expression optimization alpha-amylase (AmyS) secretion
Bacillus subtilis 1A751p
1191.6 U/mL
Amylase activity assay
PaperFu G, Liu J L, Li J S, et al. Systematic Screening of Optimal Signal Peptides for Secretory Production of Heterologous Proteins in Bacillus subtilis[J]. Journal of Agricultural and Food Chemistry, 2018, 66(50): 13141-13151. PMID30463403 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/30463403/
RECORD: Rec_0328603 VERIFIED: YES
Provenance & Taxonomy
Bacillus subtilis [Bacterium]
Tat signal peptide
1423
Structure & Mods
linear signal peptide [Linear]
canonical L-amino-acid peptide [L]
Bio-Activity Profile
Protein secretion signal peptide Secretory expression optimization Lipase (LipS) secretion
Bacillus subtilis WB800
0.00331 U/mL
Lipase activity assay
PaperZhou Y, Xu G, Yang L, et al. Effect of signal Peptide Optimization on Lipase LipS secretion and expression in Bacillus subtilis system[J]. China Biotechnology, 2015, 35(9): 42-49. (in Chinese)