Peptide Database

Peptide NameypuA signal peptide

Function Ontology

Annotation / Sequence feature ▸ Signal peptide

Biotechnology / Production and expression ▸ Secretory enzyme production ▸ Cutinase secretion

Biotechnology / Production and expression ▸ Secretory expression optimization

Standard StatusStandard

Sequence Length26 amino acids

Chemical FormulaC130H224N30O30S3

DescriptionStandard

        MKKIWIGMLAAAVLLLMVPKVSLADA
    

Molecular Weight2783.55 Da

Isoelectric Point (pI)9.70

Net Charge (pH 7.0)1.50

Net Charge (pH 7.4) 1.28

Charge Density (pH 7.0) 0.0576

GRAVY Index1.40

Hydrophobic Moment 0.25

Boman Index0.47

Instability Index7.23

Aliphatic Index 157.69

Aromaticity 0.0385

Extinction Coeff. Reduced 5500.00

Extinction Coeff. Oxidized 5500.00

The radar chart summarizes major physicochemical dimensions for quick comparison, while exact numerical values remain listed on the left.

Amino Acid Composition

TPSA 933.41

LogP -1.12

H-bond Donors 32.0

H-bond Acceptors 35.0

Rotatable Bonds 93.0

Heavy Atoms 193.0

Ring Count 3.0

Amide Bonds 25.0

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber in the current view mode. Use the buttons to zoom.

2D CHEMICAL STRUCTURE

Drag to move

Drag to move. Use the buttons to zoom.

RECORD: Rec_0309026 VERIFIED: YES

Provenance & Taxonomy

OrganismBacillus subtilis [Bacterium]

FamilySec signal peptide

Tax ID1423

Structure & Mods

Configlinear signal peptide [Linear]

Chiralitycanonical L-amino-acid peptide [L]

Bio-Activity Profile

Function

Protein secretion signal peptide Secretory expression optimization Cutinase secretion

TargetBacillus subtilis TEB1030

Activity0.29 U/mL

MethodCutinase activity assay

PaperBrockmeier U, Caspers M, Freudl R, et al. Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria[J]. Journal of Molecular Biology, 2006, 362(3): 393-402. PMID16930615 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/16930615/