Peptide Database

Peptide NameCwp signal peptide

Function Ontology

Annotation / Sequence feature ▸ Signal peptide

Biotechnology / Production and expression ▸ Secretory enzyme production ▸ Alkaline protease secretion

Biotechnology / Production and expression ▸ Secretory expression optimization

Standard StatusStandard

Sequence Length23 amino acids

Chemical FormulaC106H182N26O28S2

DescriptionStandard

        MKKVVNSVLASALALTVAPMAFA
    

Molecular Weight2332.87 Da

Isoelectric Point (pI)10.00

Net Charge (pH 7.0)1.50

Net Charge (pH 7.4) 1.28

Charge Density (pH 7.0) 0.0651

GRAVY Index1.32

Hydrophobic Moment 0.45

Boman Index0.42

Instability Index16.33

Aliphatic Index 127.39

Aromaticity 0.0435

Extinction Coeff. Reduced 0.00

Extinction Coeff. Oxidized 0.00

The radar chart summarizes major physicochemical dimensions for quick comparison, while exact numerical values remain listed on the left.

Amino Acid Composition

TPSA 850.55

LogP -6.30

H-bond Donors 29.0

H-bond Acceptors 32.0

Rotatable Bonds 75.0

Heavy Atoms 162.0

Ring Count 2.0

Amide Bonds 23.0

3D PDB MODEL

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2D CHEMICAL STRUCTURE

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RECORD: Rec_0117333 VERIFIED: YES

Provenance & Taxonomy

OrganismBacillus pumilus [Bacterium]

FamilyTat signal peptide

Tax ID1408

Structure & Mods

Configlinear signal peptide [Linear]

Chiralitycanonical L-amino-acid peptide [L]

Bio-Activity Profile

Function

Protein secretion signal peptide Secretory expression optimization Alkalineprotease (alk) secretion

TargetBacillus subtilis WB600

Activity1299.62 U/mL

MethodProtease activity assay

PaperLiu Y, Shi C, Li D, et al. Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide[J]. International journal of biological macromolecules, 2019, 138: 903-911. PMID31356949 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31356949/