Peptide Database

Peptide Namemfd signal peptide

Function Ontology

Annotation / Sequence feature ▸ Signal peptide

Biotechnology / Production and expression ▸ Secretory enzyme production ▸ Alkaline protease secretion

Biotechnology / Production and expression ▸ Secretory expression optimization

Standard StatusStandard

Sequence Length47 amino acids

Chemical FormulaC222H358N60O73S

DescriptionStandard

        MDNIQTFIKESDDFKSIINGLHEGLKEQLLAGLSGSARSVFTSALAN
    

Molecular Weight5067.64 Da

Isoelectric Point (pI)4.95

Net Charge (pH 7.0)-2.40

Net Charge (pH 7.4) -2.68

Charge Density (pH 7.0) -0.0511

GRAVY Index-0.07

Hydrophobic Moment 0.62

Boman Index0.09

Instability Index27.69

Aliphatic Index 97.66

Aromaticity 0.0638

Extinction Coeff. Reduced 0.00

Extinction Coeff. Oxidized 0.00

The radar chart summarizes major physicochemical dimensions for quick comparison, while exact numerical values remain listed on the left.

Amino Acid Composition

TPSA 2171.65

LogP -24.59

H-bond Donors 74.0

H-bond Acceptors 73.0

Rotatable Bonds 176.0

Heavy Atoms 356.0

Ring Count 4.0

Amide Bonds 51.0

3D PDB MODEL

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2D CHEMICAL STRUCTURE

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RECORD: Rec_0304123 VERIFIED: YES

Provenance & Taxonomy

OrganismBacillus subtilis subsp. subtilis str. 168 [Bacterium]

FamilyTat signal peptide

Tax ID224308

Structure & Mods

Configlinear signal peptide [Linear]

Chiralitycanonical L-amino-acid peptide [L]

Bio-Activity Profile

Function

Protein secretion signal peptide Secretory expression optimization Alkalineprotease (alk) secretion

TargetBacillus amyloliquefaciens 111018

Activity11600.9 U/mL

MethodProtease activity assay

PaperLiu Y, Shi C, Li D, et al. Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide[J]. International journal of biological macromolecules, 2019, 138: 903-911. PMID31356949 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31356949/