Peptide Database

Peptide NamecoiA signal peptide

Function Ontology

Annotation / Sequence feature ▸ Signal peptide

Biotechnology / Production and expression ▸ Secretory enzyme production ▸ Alkaline protease secretion

Biotechnology / Production and expression ▸ Secretory expression optimization

Standard StatusStandard

Sequence Length41 amino acids

Chemical FormulaC212H337N65O49S3

DescriptionStandard

        MFHLLGAQQNQKLKRRRFFCPVCGGELAVKLGLQKAPHFAH
    

Molecular Weight4676.54 Da

Isoelectric Point (pI)10.47

Net Charge (pH 7.0)5.74

Net Charge (pH 7.4) 5.34

Charge Density (pH 7.0) 0.1400

GRAVY Index-0.20

Hydrophobic Moment 0.46

Boman Index-0.01

Instability Index58.14

Aliphatic Index 80.98

Aromaticity 0.0976

Extinction Coeff. Reduced 0.00

Extinction Coeff. Oxidized 125.00

The radar chart summarizes major physicochemical dimensions for quick comparison, while exact numerical values remain listed on the left.

Amino Acid Composition

TPSA 1838.31

LogP -14.41

H-bond Donors 64.0

H-bond Acceptors 61.0

Rotatable Bonds 157.0

Heavy Atoms 329.0

Ring Count 9.0

Amide Bonds 45.0

3D PDB MODEL

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2D CHEMICAL STRUCTURE

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RECORD: Rec_0317582 VERIFIED: YES

Provenance & Taxonomy

OrganismBacillus subtilis subsp. subtilis str. 168 [Bacterium]

FamilyTat signal peptide

Tax ID224308

Structure & Mods

Configlinear signal peptide [Linear]

Chiralitycanonical L-amino-acid peptide [L]

Bio-Activity Profile

Function

Protein secretion signal peptide Secretory expression optimization Alkalineprotease (alk) secretion

TargetBacillus amyloliquefaciens 111018

Activity11266.0 U/mL

MethodProtease activity assay

PaperLiu Y, Shi C, Li D, et al. Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide[J]. International journal of biological macromolecules, 2019, 138: 903-911. PMID31356949 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31356949/