Peptide Database

Peptide NamebifL signal peptide

Function Ontology

Annotation / Sequence feature ▸ Signal peptide

Biotechnology / Production and expression ▸ Secretory enzyme production ▸ Alkaline protease secretion

Biotechnology / Production and expression ▸ Secretory expression optimization

Standard StatusStandard

Sequence Length51 amino acids

Chemical FormulaC238H402N62O69S2

DescriptionStandard

        MLQAENIKKAYGKKTIVKGISFSLKKGESFGLLGPNGAGKSTTISMISGLV
    

Molecular Weight5300.24 Da

Isoelectric Point (pI)10.10

Net Charge (pH 7.0)5.50

Net Charge (pH 7.4) 5.26

Charge Density (pH 7.0) 0.1078

GRAVY Index0.06

Hydrophobic Moment 0.53

Boman Index0.14

Instability Index6.95

Aliphatic Index 93.73

Aromaticity 0.0588

Extinction Coeff. Reduced 1490.00

Extinction Coeff. Oxidized 1490.00

The radar chart summarizes major physicochemical dimensions for quick comparison, while exact numerical values remain listed on the left.

Amino Acid Composition

TPSA 2123.86

LogP -22.16

H-bond Donors 74.0

H-bond Acceptors 77.0

Rotatable Bonds 188.0

Heavy Atoms 371.0

Ring Count 4.0

Amide Bonds 53.0

3D PDB MODEL

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2D CHEMICAL STRUCTURE

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RECORD: Rec_0333714 VERIFIED: YES

Provenance & Taxonomy

OrganismBacillus subtilis subsp. subtilis str. 168 [Bacterium]

FamilyTat signal peptide

Tax ID224308

Structure & Mods

Configlinear signal peptide [Linear]

Chiralitycanonical L-amino-acid peptide [L]

Bio-Activity Profile

Function

Protein secretion signal peptide Secretory expression optimization Alkalineprotease (alk) secretion

TargetBacillus amyloliquefaciens 111018

Activity477.8 U/mL

MethodProtease activity assay

PaperLiu Y, Shi C, Li D, et al. Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide[J]. International journal of biological macromolecules, 2019, 138: 903-911. PMID31356949 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31356949/