Peptide Database

Peptide NameyhcM signal peptide

Function Ontology

Annotation / Sequence feature ▸ Signal peptide

Biotechnology / Production and expression ▸ Secretory enzyme production ▸ Alkaline protease secretion

Biotechnology / Production and expression ▸ Secretory expression optimization

Standard StatusStandard

Sequence Length33 amino acids

Chemical FormulaC162H281N49O43S2

DescriptionStandard

        MLFNQRRGISPAALIIGSTMLITALSPQIRQRI
    

Molecular Weight3667.40 Da

Isoelectric Point (pI)12.00

Net Charge (pH 7.0)3.50

Net Charge (pH 7.4) 3.28

Charge Density (pH 7.0) 0.1061

GRAVY Index0.44

Hydrophobic Moment 0.52

Boman Index0.12

Instability Index63.76

Aliphatic Index 127.27

Aromaticity 0.0303

Extinction Coeff. Reduced 0.00

Extinction Coeff. Oxidized 0.00

The radar chart summarizes major physicochemical dimensions for quick comparison, while exact numerical values remain listed on the left.

Amino Acid Composition

TPSA 1498.05

LogP -14.08

H-bond Donors 53.0

H-bond Acceptors 49.0

Rotatable Bonds 123.0

Heavy Atoms 256.0

Ring Count 3.0

Amide Bonds 36.0

3D PDB MODEL

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2D CHEMICAL STRUCTURE

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RECORD: Rec_0260872 VERIFIED: YES

Provenance & Taxonomy

OrganismBacillus subtilis subsp. subtilis str. 168 [Bacterium]

FamilyTat signal peptide

Tax ID224308

Structure & Mods

Configlinear signal peptide [Linear]

Chiralitycanonical L-amino-acid peptide [L]

Bio-Activity Profile

Function

Protein secretion signal peptide Secretory expression optimization Alkalineprotease (alk) secretion

TargetBacillus amyloliquefaciens 111018

Activity8848.8 U/mL

MethodProtease activity assay

PaperLiu Y, Shi C, Li D, et al. Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide[J]. International journal of biological macromolecules, 2019, 138: 903-911. PMID31356949 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31356949/