Peptide Database

Peptide NameylaE signal peptide

Function Ontology

Annotation / Sequence feature ▸ Signal peptide

Biotechnology / Production and expression ▸ Secretory enzyme production ▸ Cutinase secretion

Biotechnology / Production and expression ▸ Secretory expression optimization

Standard StatusStandard

Sequence Length31 amino acids

Chemical FormulaC141H237N35O41S3

DescriptionStandard

        MKKTFVKKAMLTTAAMTSAALLTFGPDAASA
    

Molecular Weight3174.80 Da

Isoelectric Point (pI)10.00

Net Charge (pH 7.0)2.50

Net Charge (pH 7.4) 2.28

Charge Density (pH 7.0) 0.0806

GRAVY Index0.49

Hydrophobic Moment 0.31

Boman Index0.21

Instability Index13.35

Aliphatic Index 72.90

Aromaticity 0.0645

Extinction Coeff. Reduced 0.00

Extinction Coeff. Oxidized 0.00

The radar chart summarizes major physicochemical dimensions for quick comparison, while exact numerical values remain listed on the left.

Amino Acid Composition

TPSA 1210.52

LogP -12.34

H-bond Donors 43.0

H-bond Acceptors 47.0

Rotatable Bonds 105.0

Heavy Atoms 220.0

Ring Count 3.0

Amide Bonds 30.0

3D PDB MODEL

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2D CHEMICAL STRUCTURE

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RECORD: Rec_0320372 VERIFIED: YES

Provenance & Taxonomy

OrganismBacillus subtilis [Bacterium]

FamilySec signal peptide

Tax ID1423

Structure & Mods

Configlinear signal peptide [Linear]

Chiralitycanonical L-amino-acid peptide [L]

Bio-Activity Profile

Function

Protein secretion signal peptide Secretory expression optimization Cutinase secretion

TargetBacillus subtilis TEB1030

Activity1.47 U/mL

MethodCutinase activity assay

PaperBrockmeier U, Caspers M, Freudl R, et al. Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria[J]. Journal of Molecular Biology, 2006, 362(3): 393-402. PMID16930615 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/16930615/