Peptide Database

Peptide NamesbcC signal peptide

Function Ontology

Annotation / Sequence feature ▸ Signal peptide

Biotechnology / Production and expression ▸ Secretory enzyme production ▸ Alkaline protease secretion

Biotechnology / Production and expression ▸ Secretory expression optimization

Standard StatusStandard

Sequence Length48 amino acids

Chemical FormulaC224H355N57O68S2

DescriptionStandard

        MKPIALSIKGLHSFREEQTIDFEGLSGAGVFGIFGPTGSGKSSILDAM
    

Molecular Weight4998.69 Da

Isoelectric Point (pI)5.54

Net Charge (pH 7.0)-1.40

Net Charge (pH 7.4) -1.68

Charge Density (pH 7.0) -0.0293

GRAVY Index0.19

Hydrophobic Moment 0.44

Boman Index0.22

Instability Index14.87

Aliphatic Index 85.42

Aromaticity 0.0833

Extinction Coeff. Reduced 0.00

Extinction Coeff. Oxidized 0.00

The radar chart summarizes major physicochemical dimensions for quick comparison, while exact numerical values remain listed on the left.

Amino Acid Composition

TPSA 1973.51

LogP -20.00

H-bond Donors 68.0

H-bond Acceptors 70.0

Rotatable Bonds 168.0

Heavy Atoms 351.0

Ring Count 7.0

Amide Bonds 48.0

3D PDB MODEL

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2D CHEMICAL STRUCTURE

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RECORD: Rec_0340842 VERIFIED: YES

Provenance & Taxonomy

OrganismBacillus subtilis subsp. subtilis str. 168 [Bacterium]

FamilyTat signal peptide

Tax ID224308

Structure & Mods

Configlinear signal peptide [Linear]

Chiralitycanonical L-amino-acid peptide [L]

Bio-Activity Profile

Function

Protein secretion signal peptide Secretory expression optimization Alkalineprotease (alk) secretion

TargetBacillus amyloliquefaciens 111018

Activity11034.0 U/mL

MethodProtease activity assay

PaperLiu Y, Shi C, Li D, et al. Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide[J]. International journal of biological macromolecules, 2019, 138: 903-911. PMID31356949 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31356949/