Peptide Database

Peptide NameMag(i+4)15(E19K)

Function Ontology

Antimicrobial

Antimicrobial ▸ Antibacterial

Toxicity / Safety ▸ Blood cell toxicity ▸ Hemolysis ▸ Hemolytic

Standard StatusStandard

Sequence Length23 amino acids

Chemical FormulaC115H185N31O27S

DescriptionStandard

        GIGKFLHSAKKFGKAFVGKIMNS
    

Molecular Weight2465.96 Da

Isoelectric Point (pI)10.60

Net Charge (pH 7.0)4.84

Net Charge (pH 7.4) 4.58

Charge Density (pH 7.0) 0.2105

GRAVY Index0.07

Hydrophobic Moment 0.69

Boman Index0.14

Instability Index-12.58

Aliphatic Index 72.17

Aromaticity 0.1304

Extinction Coeff. Reduced 0.00

Extinction Coeff. Oxidized 0.00

The radar chart summarizes major physicochemical dimensions for quick comparison, while exact numerical values remain listed on the left.

Amino Acid Composition

TPSA 945.85

LogP -7.62

H-bond Donors 33.0

H-bond Acceptors 34.0

Rotatable Bonds 87.0

Heavy Atoms 174.0

Ring Count 4.0

Amide Bonds 23.0

3D PDB MODEL

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2D CHEMICAL STRUCTURE

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RECORD: Rec_0033043 VERIFIED: YES

Provenance & Taxonomy

Organismsynthetic construct [Synthetic]

FamilyMagainin

Tax ID32630

Structure & Mods

Confighydrocarbon-stapled side-chain cyclic peptide [Cyclic]

Chiralitycanonical L-amino-acid peptide [L]

N-TermNone

C-TermAmidation

Other ModsThe Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. The J (position: 21) in sequence is norlercine. Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling

Bio-Activity Profile

Function

Antimicrobial Antibacterial

TargetBacillus cereus ATCC 14579

Activity5.21 μg/mL

MethodMIC

PaperRida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky. Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice. Nat Biotechnol. 2019 Oct;37(10):1186-1197. Epub 2019 Aug 19. PMID31427820 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31427820/

RECORD: Rec_0100163 VERIFIED: YES

Provenance & Taxonomy

Organismsynthetic construct [Synthetic]

FamilyMagainin

Tax ID32630

Structure & Mods

Confighydrocarbon-stapled side-chain cyclic peptide [Cyclic]

Chiralitycanonical L-amino-acid peptide [L]

N-TermNone

C-TermAmidation

Other ModsThe Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. The J (position: 21) in sequence is norlercine. Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling

Bio-Activity Profile

Function

Antimicrobial Antibacterial

TargetStaphylococcus aureus ATCC 25923

Activity25.00 μg/mL

MethodMIC

PaperRida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky. Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice. Nat Biotechnol. 2019 Oct;37(10):1186-1197. Epub 2019 Aug 19. PMID31427820 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31427820/

RECORD: Rec_0208778 VERIFIED: YES

Provenance & Taxonomy

Organismsynthetic construct [Synthetic]

FamilyMagainin

Tax ID32630

Structure & Mods

Confighydrocarbon-stapled side-chain cyclic peptide [Cyclic]

Chiralitycanonical L-amino-acid peptide [L]

N-TermNone

C-TermAmidation

Other ModsThe Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. The J (position: 21) in sequence is norlercine. Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling

Bio-Activity Profile

Function

Antimicrobial Antibacterial

TargetEscherichia coli ATCC 25922

Activity1.60 μg/mL

MethodMIC

PaperRida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky. Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice. Nat Biotechnol. 2019 Oct;37(10):1186-1197. Epub 2019 Aug 19. PMID31427820 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31427820/

RECORD: Rec_0278149 VERIFIED: YES

Provenance & Taxonomy

Organismsynthetic construct [Synthetic]

FamilyMagainin

Tax ID32630

Structure & Mods

Confighydrocarbon-stapled side-chain cyclic peptide [Cyclic]

Chiralitycanonical L-amino-acid peptide [L]

N-TermNone

C-TermAmidation

Other ModsThe Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. The J (position: 21) in sequence is norlercine. Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling

Bio-Activity Profile

Function

Hemolytic

Targethuman red blood cells

Activity25 μg/mL

MethodHemolysis

PaperRida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky. Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice. Nat Biotechnol. 2019 Oct;37(10):1186-1197. Epub 2019 Aug 19. PMID31427820 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31427820/

RECORD: Rec_0322097 VERIFIED: YES

Provenance & Taxonomy

Organismsynthetic construct [Synthetic]

FamilyMagainin

Tax ID32630

Structure & Mods

Confighydrocarbon-stapled side-chain cyclic peptide [Cyclic]

Chiralitycanonical L-amino-acid peptide [L]

N-TermNone

C-TermAmidation

Other ModsThe Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. The J (position: 21) in sequence is norlercine. Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling

Bio-Activity Profile

Function

Antimicrobial Antibacterial

TargetPseudomonas aeruginosa ATCC 27853

Activity2.23 μg/mL

MethodMIC

PaperRida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky. Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice. Nat Biotechnol. 2019 Oct;37(10):1186-1197. Epub 2019 Aug 19. PMID31427820 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31427820/