Peptide Database

Peptide NamemntA signal peptide

Function Ontology

Annotation / Sequence feature ▸ Signal peptide

Biotechnology / Production and expression ▸ Secretory expression optimization

Enzyme / Catalytic activity ▸ Protease ▸ Bacillus protease novo type (BPN') secretion

Enzyme / Catalytic activity ▸ Transferase ▸ Kinase

Standard StatusStandard

Sequence Length27 amino acids

Chemical FormulaC123H212N32O35S2

DescriptionStandard

        MKWKQTLAIAAALILILAAGCSSKSSS
    

Molecular Weight2763.32 Da

Isoelectric Point (pI)9.79

Net Charge (pH 7.0)2.49

Net Charge (pH 7.4) 2.25

Charge Density (pH 7.0) 0.0921

GRAVY Index0.84

Hydrophobic Moment 0.24

Boman Index0.30

Instability Index54.05

Aliphatic Index 123.33

Aromaticity 0.0370

Extinction Coeff. Reduced 5500.00

Extinction Coeff. Oxidized 5500.00

The radar chart summarizes major physicochemical dimensions for quick comparison, while exact numerical values remain listed on the left.

Amino Acid Composition

TPSA 1078.24

LogP -10.52

H-bond Donors 40.0

H-bond Acceptors 40.0

Rotatable Bonds 94.0

Heavy Atoms 192.0

Ring Count 2.0

Amide Bonds 27.0

3D PDB MODEL

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2D CHEMICAL STRUCTURE

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RECORD: Rec_0059803 VERIFIED: YES

Provenance & Taxonomy

OrganismBacillus licheniformis DSM 13 = ATCC 14580 [Bacterium]

FamilySec signal peptide

Tax ID279010

Structure & Mods

Configlinear signal peptide [Linear]

Chiralitycanonical L-amino-acid peptide [L]

Bio-Activity Profile

Function

Protein secretion signal peptide Secretory expression optimization Bacillus protease novo type (BPN') secretion

TargetBacillus licheniformis △0F-3

Activity0.71 U/mL

MethodProtease activity assay

PaperDegering C, Eggert T, Puls M, et al. Optimization of Protease Secretion in Bacillus subtilis and Bacillus licheniformis by Screening of Homologous and Heterologous Signal Peptides[J]. Applied and Environmental Microbiology, 2010, 76(19): 6370-6376. PMID20709850 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/20709850/

RECORD: Rec_0309119 VERIFIED: YES

Provenance & Taxonomy

OrganismBacillus licheniformis DSM 13 = ATCC 14580 [Bacterium]

FamilySec signal peptide

Tax ID279010

Structure & Mods

Configlinear signal peptide [Linear]

Chiralitycanonical L-amino-acid peptide [L]

Bio-Activity Profile

Function

Protein secretion signal peptide Secretory expression optimization Bacillus protease novo type (BPN') secretion

TargetBacillus subtilis TEB1030

Activity1.01 U/mL

MethodProtease activity assay

PaperDegering C, Eggert T, Puls M, et al. Optimization of Protease Secretion in Bacillus subtilis and Bacillus licheniformis by Screening of Homologous and Heterologous Signal Peptides[J]. Applied and Environmental Microbiology, 2010, 76(19): 6370-6376. PMID20709850 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/20709850/

RECORD: Rec_0423011 VERIFIED: YES

Provenance & Taxonomy

OrganismBacillus licheniformis WX-02 [Bacterium]

FamilyTat signal peptide

Tax ID1126218

Structure & Mods

Configlinear signal peptide [Linear]

Chiralitycanonical L-amino-acid peptide [L]

Bio-Activity Profile

Function

Protein secretion signal peptide Secretory expression optimization Nattokinase secretion

TargetBacillus licheniformis △0F-3

Activity3.57 FU/mL

MethodFibrinolytic activity assay