Peptide Database

Peptide Nameyybn signal peptide

Function Ontology

Annotation / Sequence feature ▸ Signal peptide

Biotechnology / Production and expression ▸ Secretory enzyme production ▸ Cutinase secretion

Biotechnology / Production and expression ▸ Secretory expression optimization

Enzyme modulation ▸ Enzyme inhibition ▸ Amylase inhibition ▸ Alpha-amylase inhibitory

Standard StatusStandard

Sequence Length30 amino acids

Chemical FormulaC154H249N39O38S

DescriptionStandard

        MNKFLKSNFRFLLAAALGISLLASSNFIKA
    

Molecular Weight3286.93 Da

Isoelectric Point (pI)11.26

Net Charge (pH 7.0)3.50

Net Charge (pH 7.4) 3.28

Charge Density (pH 7.0) 0.1166

GRAVY Index0.79

Hydrophobic Moment 0.68

Boman Index0.27

Instability Index5.47

Aliphatic Index 120.67

Aromaticity 0.1333

Extinction Coeff. Reduced 0.00

Extinction Coeff. Oxidized 0.00

The radar chart summarizes major physicochemical dimensions for quick comparison, while exact numerical values remain listed on the left.

Amino Acid Composition

TPSA 1257.37

LogP -9.47

H-bond Donors 44.0

H-bond Acceptors 43.0

Rotatable Bonds 112.0

Heavy Atoms 232.0

Ring Count 4.0

Amide Bonds 32.0

3D PDB MODEL

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2D CHEMICAL STRUCTURE

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RECORD: Rec_0029661 VERIFIED: YES

Provenance & Taxonomy

OrganismBacillus subtilis [Bacterium]

FamilySec signal peptide

Tax ID1423

Structure & Mods

Configlinear signal peptide [Linear]

Chiralitycanonical L-amino-acid peptide [L]

Bio-Activity Profile

Function

Protein secretion signal peptide Secretory expression optimization alpha-amylase (AmyS) secretion

TargetBacillus subtilis 1A751

Activity96.1 U/mL

MethodAmylase activity assay

RECORD: Rec_0268835 VERIFIED: YES

Provenance & Taxonomy

OrganismBacillus subtilis [Bacterium]

FamilySec signal peptide

Tax ID1423

Structure & Mods

Configlinear signal peptide [Linear]

Chiralitycanonical L-amino-acid peptide [L]

Bio-Activity Profile

Function

Protein secretion signal peptide Secretory expression optimization Cutinase secretion

TargetBacillus subtilis TEB1030

Activity0.0 U/mL

MethodCutinase activity assay

PaperBrockmeier U, Caspers M, Freudl R, et al. Systematic screening of all signal peptides from Bacillus subtilis: A powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria[J]. Journal of Molecular Biology, 2006, 362(3): 393-402. PMID16930615 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/16930615/