Peptide Database

Peptide NamerpsD signal peptide

Function Ontology

Annotation / Sequence feature ▸ Signal peptide

Biotechnology / Production and expression ▸ Secretory enzyme production ▸ Alkaline protease secretion

Biotechnology / Production and expression ▸ Secretory expression optimization

Standard StatusStandard

Sequence Length33 amino acids

Chemical FormulaC165H269N49O46S

DescriptionStandard

        MARYTGPSWKLSRRLGISLSGTGKELEKRPYAP
    

Molecular Weight3707.27 Da

Isoelectric Point (pI)10.53

Net Charge (pH 7.0)4.50

Net Charge (pH 7.4) 4.27

Charge Density (pH 7.0) 0.1364

GRAVY Index-0.79

Hydrophobic Moment 0.53

Boman Index-0.18

Instability Index50.90

Aliphatic Index 65.15

Aromaticity 0.0909

Extinction Coeff. Reduced 8480.00

Extinction Coeff. Oxidized 8480.00

The radar chart summarizes major physicochemical dimensions for quick comparison, while exact numerical values remain listed on the left.

Amino Acid Composition

TPSA 1546.04

LogP -15.50

H-bond Donors 57.0

H-bond Acceptors 52.0

Rotatable Bonds 121.0

Heavy Atoms 261.0

Ring Count 7.0

Amide Bonds 32.0

3D PDB MODEL

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2D CHEMICAL STRUCTURE

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RECORD: Rec_0306194 VERIFIED: YES

Provenance & Taxonomy

OrganismBacillus subtilis subsp. subtilis str. 168 [Bacterium]

FamilyTat signal peptide

Tax ID224308

Structure & Mods

Configlinear signal peptide [Linear]

Chiralitycanonical L-amino-acid peptide [L]

Bio-Activity Profile

Function

Protein secretion signal peptide Secretory expression optimization Alkalineprotease (alk) secretion

TargetBacillus amyloliquefaciens 111018

Activity10492.4 U/mL

MethodProtease activity assay

PaperLiu Y, Shi C, Li D, et al. Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide[J]. International journal of biological macromolecules, 2019, 138: 903-911. PMID31356949 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31356949/