Peptide Database

Peptide NamethiO signal peptide

Function Ontology

Annotation / Sequence feature ▸ Signal peptide

Biotechnology / Production and expression ▸ Secretory enzyme production ▸ Alkaline protease secretion

Biotechnology / Production and expression ▸ Secretory expression optimization

Standard StatusStandard

Sequence Length48 amino acids

Chemical FormulaC216H347N61O67S2

DescriptionStandard

        MKRHYEAVVIGGGIIGSAIAYYLAKENKNTALFESGTMGGRTTSAAAG
    

Molecular Weight4934.56 Da

Isoelectric Point (pI)9.30

Net Charge (pH 7.0)1.59

Net Charge (pH 7.4) 1.31

Charge Density (pH 7.0) 0.0331

GRAVY Index0.03

Hydrophobic Moment 0.44

Boman Index0.15

Instability Index42.10

Aliphatic Index 77.50

Aromaticity 0.0833

Extinction Coeff. Reduced 4470.00

Extinction Coeff. Oxidized 4470.00

The radar chart summarizes major physicochemical dimensions for quick comparison, while exact numerical values remain listed on the left.

Amino Acid Composition

TPSA 2061.94

LogP -23.70

H-bond Donors 74.0

H-bond Acceptors 72.0

Rotatable Bonds 165.0

Heavy Atoms 346.0

Ring Count 5.0

Amide Bonds 49.0

3D PDB MODEL

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2D CHEMICAL STRUCTURE

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RECORD: Rec_0049468 VERIFIED: YES

Provenance & Taxonomy

OrganismBacillus subtilis subsp. subtilis str. 168 [Bacterium]

FamilyTat signal peptide

Tax ID224308

Structure & Mods

Configlinear signal peptide [Linear]

Chiralitycanonical L-amino-acid peptide [L]

Bio-Activity Profile

Function

Protein secretion signal peptide Secretory expression optimization Alkalineprotease (alk) secretion

TargetBacillus amyloliquefaciens 111018

Activity8632.3 U/mL

MethodProtease activity assay

PaperLiu Y, Shi C, Li D, et al. Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide[J]. International journal of biological macromolecules, 2019, 138: 903-911. PMID31356949 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31356949/