Peptide Database

Peptide NameMag(i+4)15(G3K)

Function Ontology

Antimicrobial

Antimicrobial ▸ Antibacterial

Toxicity / Safety ▸ Blood cell toxicity ▸ Hemolysis ▸ Hemolytic

Standard StatusStandard

Sequence Length23 amino acids

Chemical FormulaC118H189N31O29S

DescriptionStandard

        GIKKFLHSAKKFGKAFVGEIMNS
    

Molecular Weight2538.02 Da

Isoelectric Point (pI)10.18

Net Charge (pH 7.0)3.85

Net Charge (pH 7.4) 3.58

Charge Density (pH 7.0) 0.1672

GRAVY Index-0.07

Hydrophobic Moment 0.74

Boman Index0.09

Instability Index-0.10

Aliphatic Index 72.17

Aromaticity 0.1304

Extinction Coeff. Reduced 0.00

Extinction Coeff. Oxidized 0.00

The radar chart summarizes major physicochemical dimensions for quick comparison, while exact numerical values remain listed on the left.

Amino Acid Composition

TPSA 983.15

LogP -7.39

H-bond Donors 34.0

H-bond Acceptors 35.0

Rotatable Bonds 90.0

Heavy Atoms 179.0

Ring Count 4.0

Amide Bonds 23.0

3D PDB MODEL

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2D CHEMICAL STRUCTURE

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RECORD: Rec_0129370 VERIFIED: YES

Provenance & Taxonomy

Organismsynthetic construct [Synthetic]

FamilyMagainin

Tax ID32630

Structure & Mods

Confighydrocarbon-stapled side-chain cyclic peptide [Cyclic]

Chiralitycanonical L-amino-acid peptide [L]

N-TermNone

C-TermAmidation

Other ModsThe Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. The J (position: 21) in sequence is norlercine. Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling

Bio-Activity Profile

Function

Hemolytic

Targethuman red blood cells

Activity25 μg/mL

MethodHemolysis

PaperRida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky. Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice. Nat Biotechnol. 2019 Oct;37(10):1186-1197. Epub 2019 Aug 19. PMID31427820 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31427820/

RECORD: Rec_0146094 VERIFIED: YES

Provenance & Taxonomy

Organismsynthetic construct [Synthetic]

FamilyMagainin

Tax ID32630

Structure & Mods

Confighydrocarbon-stapled side-chain cyclic peptide [Cyclic]

Chiralitycanonical L-amino-acid peptide [L]

N-TermNone

C-TermAmidation

Other ModsThe Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. The J (position: 21) in sequence is norlercine. Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling

Bio-Activity Profile

Function

Antimicrobial Antibacterial

TargetPseudomonas aeruginosa ATCC 27853

Activity3.34 μg/mL

MethodMIC

PaperRida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky. Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice. Nat Biotechnol. 2019 Oct;37(10):1186-1197. Epub 2019 Aug 19. PMID31427820 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31427820/

RECORD: Rec_0173708 VERIFIED: YES

Provenance & Taxonomy

Organismsynthetic construct [Synthetic]

FamilyMagainin

Tax ID32630

Structure & Mods

Confighydrocarbon-stapled side-chain cyclic peptide [Cyclic]

Chiralitycanonical L-amino-acid peptide [L]

N-TermNone

C-TermAmidation

Other ModsThe Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. The J (position: 21) in sequence is norlercine. Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling

Bio-Activity Profile

Function

Antimicrobial Antibacterial

TargetBacillus cereus ATCC 14579

Activity5.21 μg/mL

MethodMIC

PaperRida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky. Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice. Nat Biotechnol. 2019 Oct;37(10):1186-1197. Epub 2019 Aug 19. PMID31427820 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31427820/

RECORD: Rec_0253450 VERIFIED: YES

Provenance & Taxonomy

Organismsynthetic construct [Synthetic]

FamilyMagainin

Tax ID32630

Structure & Mods

Confighydrocarbon-stapled side-chain cyclic peptide [Cyclic]

Chiralitycanonical L-amino-acid peptide [L]

N-TermNone

C-TermAmidation

Other ModsThe Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. The J (position: 21) in sequence is norlercine. Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling

Bio-Activity Profile

Function

Antimicrobial Antibacterial

TargetStaphylococcus aureus ATCC 25923

Activity10.49 μg/mL

MethodMIC

PaperRida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky. Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice. Nat Biotechnol. 2019 Oct;37(10):1186-1197. Epub 2019 Aug 19. PMID31427820 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31427820/

RECORD: Rec_0419391 VERIFIED: YES

Provenance & Taxonomy

Organismsynthetic construct [Synthetic]

FamilyMagainin

Tax ID32630

Structure & Mods

Confighydrocarbon-stapled side-chain cyclic peptide [Cyclic]

Chiralitycanonical L-amino-acid peptide [L]

N-TermNone

C-TermAmidation

Other ModsThe Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. The J (position: 21) in sequence is norlercine. Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling

Bio-Activity Profile

Function

Antimicrobial Antibacterial

TargetEscherichia coli ATCC 25922

Activity1.60 μg/mL

MethodMIC

PaperRida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky. Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice. Nat Biotechnol. 2019 Oct;37(10):1186-1197. Epub 2019 Aug 19. PMID31427820 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/31427820/