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MYP_004714
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Basic Information
wapA signal peptide
Annotation / Sequence feature ▸ Signal peptide
Biotechnology / Production and expression ▸ Secretory expression optimization
Enzyme / Catalytic activity ▸ Lyase
Standard
28 amino acids
C155H265N45O31S2
Standard
Sequence
MKKRKRRNFKRFIAAFLVLALMISLVPA
Physicochemical Analysis
3319.18 Da
12.00
7.50
7.27
0.2677
0.45
0.77
-0.02
72.46
118.57
0.1071
0.00
0.00
The radar chart summarizes major physicochemical dimensions for quick comparison, while exact numerical values remain listed on the left.
Residue Composition
Amino Acid Composition
Chemical Descriptors
1255.23
-5.67
46.0
41.0
115.0
233.0
4.0
28.0
Structural Visualization
3D PDB MODEL
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2D CHEMICAL STRUCTURE
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Experimental Records
RECORD: Rec_0025152 VERIFIED: YES
Provenance & Taxonomy
Bacillus subtilis [Bacterium]
Tat signal peptide
1423
Structure & Mods
linear signal peptide [Linear]
canonical L-amino-acid peptide [L]
Bio-Activity Profile
Protein secretion signal peptide Secretory expression optimization Alkaline polygalacturonate lyase (PGL) secretion
Bacillus subtilis WB600
24.8 U/mL
Polygalacturonate lyase activity assay
PaperZhang J J, Kang Z, Ling Z M, et al. High-level extracellular production of alkaline polygalacturonate lyase in Bacillus subtilis with optimized regulatory elements[J]. Bioresource Technology, 2013, 146: 543-548 PMID23973973 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/23973973/
RECORD: Rec_0182194 VERIFIED: YES
Provenance & Taxonomy
Bacillus subtilis [Bacterium]
Tat signal peptide
1423
Structure & Mods
linear signal peptide [Linear]
canonical L-amino-acid peptide [L]
Bio-Activity Profile
Protein secretion signal peptide Secretory expression optimization CotA-laccase secretion Textile wastewater decolorization
Bacillus subtilis WB600
238.5 U/mL
Laccase activity assay
PaperGuan Z B, Shui Y, Song C M, et al. Efficient secretory production of CotA-laccase and its application in the decolorization and detoxification of industrial textile wastewater[J]. Environmental Science and Pollution Research, 2015, 22(12): 9515-9523. PMID25847445 Evidence URLhttps://pubmed.ncbi.nlm.nih.gov/25847445/
RECORD: Rec_0273397 VERIFIED: YES
Provenance & Taxonomy
Bacillus subtilis [Bacterium]
Tat signal peptide
1423
Structure & Mods
linear signal peptide [Linear]
canonical L-amino-acid peptide [L]
Bio-Activity Profile
Protein secretion signal peptide Secretory expression optimization L-Asparaginase secretion
Bacillus subtilis WB600
14.81 U/mL
Asparaginase activity assay
PaperXuan Chen.Study on the expression and fermentation optimization of L-asparaginase in Bacillus subtilis WB600[D]. Jiangnan University, 2015. (in Chinese)