ASSAY RECORD

MP-VB2

Vespa bicolor [Animal]

Hemolysis tested, Non-hemolytic

rat red blood cells

Hemolysis assay (320 μg/mL peptide)

-

MP-VB2

Vespa bicolor [Animal]

Mast-cell degranulation, Low degranulation activity

RBL-2H3 cells

Mast-cell degranulation EC50 (β-hexosaminidase release)

-

MP-VB2

Vespa bicolor [Animal]

Mast-cell degranulation, Low degranulation activity

P815 cells

Mast-cell degranulation assay at 80 μg/mL (β-hexosaminidase release; low activity)

-

A4

synthetic construct [Synthetic]

Non-hemolytic, Cytocompatible

human erythrocytes (blood type B)

Hemolysis assay (3.13–100 μg/mL; no significant hemolysis)

-

A4

synthetic construct [Synthetic]

Cytocompatible, Low cytotoxicity

human dermal fibroblasts (HDF)

MTT cell viability assay (6.25–100 μg/mL; high viability at 50–100 μg/mL)

-

FLP-19

Caenorhabditis elegans [Animal]

RFamide, Neuropeptide, Neuromodulator, Myotropic

Ascaris suum ovijector

In vitro ovijector contractility assay (excitatory response at 10 μM)

-

Unnamed

Mustelus griseus [Animal]

ABTS radical scavenging, Antioxidant

ABTS radical

IC50 (ABTS radical scavenging assay)

-

Unnamed

Mustelus griseus [Animal]

Superoxide radical scavenging, Antioxidant

superoxide radical

IC50 (superoxide radical scavenging assay)

-

Unnamed

Mustelus griseus [Animal]

Lipid peroxidation inhibition, Antioxidant

linoleic acid model system

Qualitative linoleic acid autooxidation inhibition assay

-

Toxin Ctri18

Chaerilus tricostatus [Animal]

Immunomodulatory, IL-2 secretion inhibition, Toxin

activated human T lymphocytes / PBMCs

ELISA (IL-2 secretion inhibition)

-

discodermin A

Discodermia kiiensis [Animal]

Development inhibition, Toxin

starfish embryos

Qualitative embryo development inhibition assay

-

discodermin A

Discodermia kiiensis [Animal]

Membrane permeabilization, Cytotoxic

A10 vascular smooth muscle cells

Qualitative membrane permeabilization assay

-

discodermin A

Discodermia kiiensis [Animal]

Hemolytic, Cytolytic

rabbit erythrocytes

Qualitative hemolysis assay

-

Glucagon I

Polyodon spathula [Animal]

Hormone, GLP1R agonism, cAMP signaling

GLP1R-transfected CHL cells

cAMP accumulation assay

-

Glucagon I

Polyodon spathula [Animal]

Hormone, GCGR agonism, cAMP signaling

GCGR-transfected HEK293 cells

cAMP accumulation assay

-

Glucagon I

Polyodon spathula [Animal]

Hormone, Glycogenolytic activity

dispersed rockfish hepatocytes

glycogenolysis assay

-

Huwentoxin-V

Ornithoctonus huwena [Animal]

Calcium channel inhibitor, Insecticidal, Neurotoxin, Toxin

Periplaneta americana dorsal unpaired median neurons; high-voltage-activated calcium channels

IC50; whole-cell patch-clamp

-

Charybdotoxin (ChTX)

Leiurus quinquestriatus hebraeus [Animal]

Potassium channel blocker, Neurotoxin, Toxin

high-conductance Ca2+-activated K+ channel in bovine aortic sarcolemmal membranes

Ki; competitive radioligand binding assay

-

Helicostatin-9

Helicoverpa armigera [Animal]

Myotropic, Neuropeptide, Inhibitory regulation of foregut peristalsis

Helicoverpa armigera crop/foregut musculature

in vitro crop musculature peristalsis assay

-

mIRBP161-180

Mus musculus [Animal]

Uveitogenic, T-cell epitope, Immunological

IRBP-specific T cells; B10.RIII mouse EAU model

experimental autoimmune uveitis induction; T-cell proliferation assay

-